Abstract

RODRIGUEZ MEDINA, Narciso N et al. Studying the diversity of guava (Psidium guajava L.) accessions using ISTR molecular markers. Rev. colomb. biotecnol [online]. 2010, vol.12, n.1, pp.113-123. ISSN 0123-3475.

Genetic variability studies can be useful for the rational management of material as well as its conservation and improvement. Inverse sequence-tagged repeat (ISTR) is a PCR-based technique which allows individuals and populations’ genetic diversity to be studied, cultivars to be identified, etc. The present work was thus aimed at using this molecular marker to study guava accession diversity. An absence-presence matrix for polymorphic bands was generated for data analysis; cluster analysis was developed from this, based on Jaccard’s coefficient and the UPGMA method for dendrogram construction using NTSYS-pc software. Guava genotype evaluation with the ISTR marker generated 52 polymorphic bands, allowing all the materials being evaluated to be differentiated, thereby corroborating this technique’s use for identifying accessions in the specie. Cluster analysis revealed the formation of four defined diversity groups and the presence of four external accessions. The differences encountered for accession clustering with ISTR regarding those previously obtained by AFLP and SSR suggested that choosing the most appropriate technique for determinate studies can be difficult. The results indicated the need for carrying out integrated studies on this crop’s germplasm bank to ensure more suitable management.

Keywords : Genotype identification; relatedness; polymorphism.

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