Abstract

DIAZ¹, Lucía Primitiva; NAMUR, Jorge Julio; BOLLATI, Sebastián Agustín  and  ARCE, Osvaldo Ernesto Antonio. Acclimatization of Phalaenopsis and Cattleya obtained by micropropagation. Rev. colomb. biotecnol [online]. 2010, vol.12, n.2, pp.27-40. ISSN 0123-3475.

The quality of micropropagated plants relies on the acclimatization stage. This research intends to develop an efficient protocol to obtain the acclimatization of Phalaenopsis and Cattleya. Plants of Phalaenopsis obtained from protocorms were selected. They came from flowering stalks grown at modified Murashige and Skoog (MS) (1962) medium and classified by growth ranks and put into moss, mesquite wood shaving and perlite (1:1:1), into a humidity chamber. The protocorms were multiplied at MS from Cattleya sown in Knudson C (1946) medium; regenerated plants of 1-2 cm were selected, and implanted in humidity chamber on: moss, coal and perlite (1:1:1) MCP; mesquite wood shavings, coal and perlite (1:1:1) ACP; moss and perlite (1:1) MP; mesquite wood shaving and perlite (1:1) AP. The following results were obtained: Phalanopsis: a) Survival: 44% in R₀ and 100% in R_I and R_II of the. b) Number of leaves: R_I gave on average 1 more leaf than the range 0; c) Roots number and length: R_I and R_II gave on average 2 more roots than R₀, and there were no significant differences in length. d) Height: R_II presented greater growth than R_I and R_o. Cattleya: a) The survival in MCP was 0%, MP 16 %, ACP 32% and AP 80%. b) The height in MP was significantly superior to the ones in ACP and AP. Plants from both genera need to achieve a 2 to 4 cm growth rank in vitro to endure the greenhouse conditions. MAP was the best substrate in Phalaenopsis and moss-perlite in Cattleya.

Keywords : Orchidaceae; substrates; in vitro culture.

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