Revista Colombiana de Biotecnología
Print version ISSN 0123-3475
COSTA, Marcia; TORRES, Marcelo; MAGARINOS, Haroldo and REYES, Alejandro. Production and partial purification of Aspergillus ficuum hydrolytic enzymes in solid state fermentation of agroindustrial residues. Rev. colomb. biotecnol [online]. 2010, vol.12, n.2, pp. 163-175. ISSN 0123-3475.
In this paper, describes the production of the enzymes phytase, cellulase, xylanase and protease by Aspergillus ficuum DSM 932 strain, in solid state fermentation (SSF) using canola cake and cranberry pomace as substrates. The enzyme activity was used in each case as an indirect measure of the enzymes production. Canola meal turned out to be a better substrate for phytase, cellulase and xylanase, while cranberry pomace was found to be a potential substrate for protease. Various ultrafiltration operations were carried out, decreasing the cut off membranes out in order to purify partially extracts of enzymes phytase, cellulase and xylanase, obtained from canola meal. Phytase was found to have a size >100 kDa, whereas cellulase and xylanase activity present in the retained 10, 30 and 50 kDa, suggesting that isozymes of both complexes have molecular weights ranging between 10 and 100 kDa.
Keywords : Solid fermentation; canola pressed cake; aspergillus ficuum; phytase; xylanase.