Revista Colombiana de Biotecnología
versión impresa ISSN 0123-3475
GARCIA-AGUILA, Leyanis; GOMEZ-KOSKY, Rafael; ALVARADO-CAPO, Yelenys y REYES, Zoe Sarría y Maritza. Effect of inoculum density on formation and morphology of plantain somatic embryos (Musa spp. AAAB, cv. Hybrid ‘FHIA-21’). Rev. colomb. biotecnol [online]. 2010, vol.12, n.2, pp. 240-247. ISSN 0123-3475.
Somatic embryogenesis is an interesting proposal for mass propagation of plants from the FHIA-21 (Musa AAAB). However, it is characterized by an asynchronous process, due to the presence of somatic embryos at different stages of ontogenetic development. Reduction of asynchrony is an important aspect to use this system of regeneration in plant propagation. Effect of inoculation density on the formation and uniform morphological development of somatic embryos of cv. ‘FHIA-21’ was determine by adding 0.5, 1.0, 1.5 and 2.0 g of fresh mass (gMF) of embryogenic cell aggregates, in Erlenmeyer flasks (250 ml) containing 30 ml liquid culture medium. Evaluations were made within 30 days of culture determining the number and length of somatic embryos (mm). Also, morphological and histological descriptions of these were analyzed. Results showed the regulatory effect of inoculation density on the formation and morphological development of somatic embryos. The biggest formation of embryos with a uniform morphological development occurred in 1.5 gMF inoculation density. This provided 86.4% of embryos with a length of 0.21 to 4.0 mm and the study of histological sections showed histological evidence of the globular stage. Synchronization during the formation and development of somatic embryos cv. FHIA-21 increased the efficiency of somatic embryogenesis.
Palabras llave : Histology; morphology; somatic embryogenesis; synchronization.