Revista Colombiana de Biotecnología
versão impressa ISSN 0123-3475
MENCHACA G., Rebeca A. et al. In vitro germination of Vanilla planifolia and V. pompona hybrids. Rev. colomb. biotecnol [online]. 2011, vol.13, n.1, pp. 80-84. ISSN 0123-3475.
The cultivation of vanilla is extensive clonal asexual propagation by cuttings and fruit production by artificial self-pollination. This feature tends to inhibit the genetic variation and the emergence of new individuals by sexual recombination. Therefore, sexual propagation between species it is considered necessary to obtain new individuals with desirable characteristics for cultivation. In this paper we were able to obtain hybrids of two species of vanilla. In general, the hybrids seeds obtained whit the higher germination percentage were interspecific crosses of V. planifolia and V. pompona (85%), followed by reverse cross V. planifolia and V. pompona (57.9%). Seeds obtained of V. pompona self-pollination were very low germination (10.8%) while those obtained from V. planifolia self-pollination showed no germination in any media culture. The most efficient medium for all treatments was the Murashige & Skoog (MS) supplemented whit 400 mg/L-1glutamine and 80 mg/L-1 of adenine sulfate.
Palavras-chave : Vanilla; breeding; vanilla hybrids; in vitro germination; Vanilla planifolia; Vanilla pompona.