Revista Colombiana de Biotecnología
versão impressa ISSN 0123-3475
GIL, José Fernando; COTES, José Miguel e MARIN, Mauricio. Incidence of potyvirus and molecular characterization of PVY in potato (Solanum tuberosum L.) growing regions of Colombia. Rev. colomb. biotecnol [online]. 2011, vol.13, n.1, pp. 85-93. ISSN 0123-3475.
Potato viruses are responsible for significant reductions in seed quality and crop yields around the world. In this study, we evaluate the levels of incidence of potyvirus in ten potato growing regions of Colombia from the provinces of Antioquia, Boyacá, Cundinamarca and Nariño. As PVY is the most limiting potyvirus in potato farming, a molecular characterization of Colombian PVY strains was also performed. Incidence was evaluated by ELISA using general potyvirus antibodies. Phylogenetic analysis were made on the partial sequence of the capsid gene from 33 isolates. A portion of the 5´ and 3' genome ends was obtained from two Colombian strains. Results confirmed the presence of potyvirus in the four provinces with an average incidence of 72%. The lowest incidence value was 56%. Molecular analysis clustered all Colombian isolates with strains PVYN and PVYNTN, the latter responsible for the disease known as PTNRD (Potato tuber necrotic ringspot disease).
Palavras-chave : Capsid; Elisa; RT-PCR; sequencing.