Revista Colombiana de Biotecnología
Print version ISSN 0123-3475
VILCHEZ, Jorge et al. Multiplicación en sistemas de inmersión temporal y enraizamiento ex vitro de ocumo blanco (Xanthosoma sagittifolium (L.) Schott). Rev. colomb. biotecnol [online]. 2011, vol.13, n.1, pp. 94-102. ISSN 0123-3475.
The white cocoyam (Xanthosoma sagittifollium (L.) Schott), is an Arácea cultivated in tropical countries, due to the nutritional value of its corms. The main limiting factor for cultivation is the lack of healthy seed, by this reason be outlined to evaluate the multiplication of shoots of white cocoyam in temporary immersion systems and the ex vitro rooting of the same. For that which, itself study, the time and frequency of immersion and the density of explants on the proliferation of the shoots. As well as, the effect of the indole acetic acid (IAA) and indole butyric acid (IBA) on ex vitro rooting the shoots was studied. According to the results obtained, the greater efficiency in the proliferation of shoots was obtained utilizing the temporary immersion system of the type RITA®, with a frequency and time of immersion of 6 times/day and 5 min, respectively and a density of 9 explantes/RITA®. In the ex vitro rooting was determined that under the conditions of employed cultivation is not necessary the use of auxins. It is concluded that is possible the efficient multiplication of white cocoyam in temporary immersion systems and to carry out the ex vitro rooting without the use of auxins.
Keywords : Acclimatation; liquid media; immersion time; immersion frecuency; RITA®.