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Revista Colombiana de Biotecnología

Print version ISSN 0123-3475


GONZALEZ VEGA, María Esther; CASTILLA VALDES, Yanelis  and  HERNANDEZ RODRIGUEZ, Annia. Obtaining of cell suspensions and somatic embryos of coffee (Coffea canephora P.) using of bacterial metabolites. Rev. colomb. biotecnol [online]. 2011, vol.13, n.1, pp.123-131. ISSN 0123-3475.

The somatic embryogenesis is important as model system to study the development of physiologic and molecular events that sustain the embryogenesis in plants, is an appropriate system for the massive propagation of vegetable species and as tool for the genetic improvement, the germplasm conservation and the validation of new biological products and to facilitate the multiplication to great scale through the culture in liquid medium, as well as application in bioreactores, providing high multiplication frequency, quick growth of the embryo, easiness of absorption of nutritious and reduction of the subculturing. In this paper the somatic embryogenesis was used to evaluate the effect of bacterial compounds in the induction of cellular suspensions and somatic embryos in three coffee genotypes of Coffea canephora P. var. Robusta. Were studied inoculo densities among 0.2, 0.5, 1.0 and 3.0 gMF/L-1 and the effect of different culture medium in the development of the process. The results showed a behavior differed in the genotype M-28, in medium culture with conventional regulators of growth and the alternatives. Strong relationship was evidenced between the cellular viability and the number of cells, in the different cultivation conditions and according to the inoculo density, a wide range of size and forms as observed in the populations of somatic embryos. The conversion percentages with the medium MDE-2, evidenced improvements of this indicator for the coffee.

Keywords : Coffea canephora; Robusta; density; germination; conversion.

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