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Revista Colombiana de Biotecnología

Print version ISSN 0123-3475

Abstract

GOMEZ LUNA, Liliana; ALVAREZ, Inaudis  and  RIVERO, Roger. Culture of Chlorella vulgaris using soy waste and applying a magnetic field Cultivo de C. vulgaris con soja y CM. Rev. colomb. biotecnol [online]. 2011, vol.13, n.2, pp.27-38. ISSN 0123-3475.

Chlorella has occupied the attention of biotechnologists to be an important source of biomass for the production of metabolites with a pharmaceutical, industrial and chemical interest; however, managing large-scale cultures, remains a process that needs to find alternatives become cheaper and viable. This paper presents an exploratory study that evaluated the feasibility of using the residual of soybeans softening, as a culture medium for the microalgae Chlorella vulgaris, yielding excellent values for maximum cell density (Kmax: 360 x 106 cells.mL-1). Subsequently assessed the effects of applying a magnetic field (MF) of 0.03T, obtaining maximum cell densities in a shorter time, which may constitute an essential base for a new culture methodology. The quality of the C. vulgaris biomass was evaluated from a biochemical point of view, determining protein concentration, carbohydrates and lipids concentration in the exponential phase of growth, whose accumulation is favored, depending on the MF application protocol, while the lipid concentration is maximum if the MF is applied during the stationary phase, with values from 1.70 ±0.02 to 3.48 ±0.03 pg cell. It would be an advantage for the management of commercial cultures of Chlorella depending on the biomass final use.

Keywords : neodymium magnet; pigments; nitrogen; biomass; microalgae.

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