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Revista Colombiana de Biotecnología

Print version ISSN 0123-3475

Abstract

JUYO, D. K.; GERENA, H. N.  and  MOSQUERA, T.. Evaluation of molecular markers associated with resistance to late blight (Phytophthora infestans L.) in diploid and tetraploid potatoes. Rev. colomb. biotecnol [online]. 2011, vol.13, n.2, pp.51-62. ISSN 0123-3475.

Potato is an important worldwide crop seriously affected by late blight disease caused by the oomycete Phytophthora infestans. Currently, the most effective way to control the disease is developing resistant cultivars to the pathogen by identifying genes that confer resistance to the pathogen. For this purpose it is important to find molecular markers associated with the trait. In this study, the SCAR (Sequence Characterized Amplified Region) markers: CosA, GP179, BA47f2 y Prp1, associated with late blight and the resistant gen R1 were evaluated in 22 tetraploid cultivars from subspecie andigena and five wild potato species. Polymorphism was evaluated and it was evaluated if polymorphic alleles allow differentiating resistant from susceptible genotypes. The fragment length for each marker was compared to the allele size reported associated to resistance. The analysis considered the presence/absence of the fragments: CosA210, CosA250, R11400, R11800, BA47f2500, GP179570, Prp1300, Prp1600 and Prp1900. The results indicated that both, tetraploid cultivars and wild potatoes, showed polymorphisms with all these markers, except with the GP179 marker. It was not found correlation between resistance and the presence of specific alleles. Evidence found in this study indicates that results obtained with molecular markers differed between subsp. tuberosum and subsp. andigena.

Keywords : Phytophthora infestans; late blight resistance; diagnostic markers; Solanum tuberosum subsp. andigena.

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