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Revista Colombiana de Biotecnología

Print version ISSN 0123-3475

Abstract

SANTOS PINO, Arletys et al. Multiplication in temporary immersion system cocoyam clone "Viequera" (Xanthosoma spp.). Rev. colomb. biotecnol [online]. 2011, vol.13, n.2, pp.97-106. ISSN 0123-3475.

Temporary Immersion System (TIS) is a alternative in the micropropagation of plants. This work was carried out to establish a protocol for the multiplication of clone TIS cocoyam "Viequera". The effect of three immersion times (7, 14 and 21 minutes), three immersion frequencies (2, 4 and 6 hours per day), four volumes of culture medium (5, 10, 15 and 20 mL per shoot initial) and four times of cultivation (15, 18, 21 and 25 days) in the multiplication of shoots from axillary buds. With immersion time of 14 minutes every 4 hours, a volume of 15 ml of culture medium for initial outbreak and 18 days of culture, achieved the best performance in the multiplication of shoots from axillary buds, with a coefficient of multiplication 10.50. The proposed protocol increases the productivity of propagated material compared to those developed in semisolid culture media, representing a reduction in production costs by introducing the increase in cultivation in commercial laboratories.

Keywords : immersion time and frequency; volume of culture medium; culture time..

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