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Revista Colombiana de Biotecnología

Print version ISSN 0123-3475

Abstract

SUAREZ CARDOSO, Oscar Manuel; NARANJO GOMEZ, Esther Julia; ATEHORTUA GARCES, Lucía  and  BLAIR TRUJILLO, Silvia. Direct organogenesis in vitro from leaves of Solanum nudum Dunal - Antiplasmodial plant. Rev. colomb. biotecnol [online]. 2011, vol.13, n.2, pp.186-192. ISSN 0123-3475.

Solanum nudum Dunal (Solanaceae) is a plant with a potential for developing chemotherapeutic treatments against malaria. This is the first report of a fast, efficient, and reproducible direct organogenesis protocol from leave segments from in vitro seed-grown plantlets. Leaves segments from 5 months old germinated plantlets were placed on half concentration Murashige and Skoog medium (MS), supplemented with several concentrations of Bencilaminopurin (BAP) combinated with Indolacetic Acid (IAA). Dark/ light incubation effect in periods 0/45, 15/30 and 30/15 dark/light days were evaluated on the buds induction. High frequency buds formation was shown (4,83) in explants cultured on MS supplemented with BAP 2,0 mg/L and AIA 0,1 mg/L under a period of 30 days of dark condition incubation. After induction, buds obtained were transferred to MS medium supplemented with gibberellic acid (GA3) 0,3 mg/L and maintained under artificial cool light, there the plantlets rooted. Regenerated plantlets were placed under greenhouse conditions and these were morphologically similar to donor plants.

Keywords : Solanaceae; Solanum nudum; Antimalarial plant; micropropagation; buds induction.

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