Resumo

SALAZAR MERCADO, Seir Antonio  e  CANCINO, Giovanni Orlando. Evaluation of the effects of two organics supplements on in vitro germination of native orchids in the province of Pamplona, Colombia. Rev. colomb. biotecnol [online]. 2012, vol.14, n.1, pp.53-59. ISSN 0123-3475.

The continued habitat destruction of native orchids in Colombia and its germination limitations in the wild has led to greater emphasis of the species conservation, therefore to preserve endangered orchids in vitro culture is an alternative tool. This research evaluated orchid seeds asymbiotic germination and seedling formation of Prosthechea vespa Vell. and Sobralia klotzscheana Rchb. f. species in Murashige and Skoog (MS) culture medium with two organic supplements (pineapple juice and coconut water). Mature capsules from P. vespa and S. klotzscheana species were collected in the Province of Pamplona, northeastern region of Colombia and their viability with tetrazolium staining was determined. The seeds were disinfected and sown by the syringe method. The seeds viability were 87,2% in P. vespa and 80,6% in S. klotzscheana. The corrected percentage of viability compared to the germination was higher, between 2,8% (P. vespa) and 0,5% (S. klotzscheana). This study showed that MS medium supplemented with pineapple juice has a greater response to asymbiotic germination and seedling formation in P. vespa (22%) and S. klotzscheana (43%) with statistically significant differences (P≤0,05: Tukey HSD).

Palavras-chave : conservation; asymbiotic germination; orchids; viability; tetrazolium.

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