Resumen

ROJAS SIERRA, Johanna Norela; PEREZ CORDERO, Alexander Francisco; MARTINEZ AVILEZ, Jose Gregorio  y  MIELES GALINDO, Jorge Ulises. Antibacterial activity of leaf extract Melia azedarach L. Rev. colomb. biotecnol [online]. 2012, vol.14, n.1, pp.224-232. ISSN 0123-3475.

The work was objective to evaluate the antibacterial activity of extracts from leaves of Melia azedarach (L) on six pathogenic bacteria. Total ethanol extract was obtained initially by extraction with method Soxhlet and from this prepared liquid-liquid fractions with petroleum ether and ethyl acetate. The total extract and the two fractions were diluted at different concentrations (ppm) to evaluate its antibacterial activity in vitro. More susceptible bacteria were the pathogenic human Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa and Klebsiella oxytoca, in relation to the plant pathogen Burkholderia glumae who showed resistance to all treatments. Pathogenic bacteria were more susceptible to the fraction of petroleum ether to the minimum concentration of 25 ppm. Screen plant phytochemical indicated presence of secondary metabolites type alkaloids, terpenes/sterols, saponins, tannins and anthocyanins. These results demonstrate the potential use of M. azederach as biological control alternatively on analysed bacterial.

Palabras clave : bacteria; pathogenic; inhibition; extract.

        · resumen en Español     · texto en Español     · Español ( pdf )