Abstract

LOPEZ CASTRO, Karen Rocío  and  FRANCO-LARA, Liliana. Presence of the transposable genetic element dTdic1 in Dianthus caryophyllus with variegated and no variegated flowers. Rev. colomb. biotecnol [online]. 2012, vol.14, n.2, pp.38-48. ISSN 0123-3475.

The objective of this work was to search for the EGT (Transposable Genetic Element) dTdic1 that has been associated with color variegation of carnation flowers and its relationship with two genes that code for enzymes involved in the synthesis of anthocyanins, Chalcona isomerase (CHI) and Dihidroflavonol reductase (DFR). Its presence and expression was evaluated in seven genotypes of variegated flowers and four no variegated flower genotypes (one hybrid line and three commercial cultivars). A high number of copies (undefined) of copies of the dTdic1 element was detected in variegaated and no variegated lines. Therefore, the main presence of this EGT was not associated directly with variegation of floral pigments. Additionally, dTdic1 was found interrupting the CHI gene in four variegated and one no variegated phenotypes. No evidence was observed of insertion of dTdic1 in the DFR gene in any of the genotypes.

Keywords : Transposons; chalcone isomerase; anthocyanins.

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