Abstract

DELGADO-PAREDES, Guillermo E. et al. Tissue culture of Piper sp. (Piperaceae): in vitro propagation, organogenesis and germplasm conservation. Rev. colomb. biotecnol [online]. 2012, vol.14, n.2, pp.49-60. ISSN 0123-3475.

In the New World the genus Piper (Piperaceae) contains at least 500 species in which many biologically active compounds were identified; is why the study of the chemistry and biosynthesis in members of this genus are of interest, and here techniques in vitro tissue culture plays a fundamental role. The aim of this work was to establish several in vitro culture systems in different Piper species in order to propagate clonally and regenerate by direct organogenesis for the establishment the field plants, the conservation and international germoplasm exchange. The seed germination achieved approximately 20 - 99% after 4 weeks of culture. The micropropagation was performed on MS culture medium supplemented with sucrose (3%), IAA (0,02 mg L^-1) and GA₃ (0,02 mg L^-1). Callus induction and morphogenetic potential of root, petiole, leaf, node and internode explants was investigated to develop a reliable plant regeneration protocol. The in vitro germplasm conservation and international germplasm exchange also was realized, between Perú, Brazil and Ecuador.

Keywords : Germplasm international exchange; growth regulators; in vitro seeds germination; morphogenesis; recalcitrant seeds.

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