Abstract

MARTINEZ MEDINA, Silvio de Jesús et al. Effect of two cytokinin, ascorbic acid and sucrose to obtain in vitro shoots of sorghum for calli formation. Rev. colomb. biotecnol [online]. 2012, vol.14, n.2, pp.101-110. ISSN 0123-3475.

The current work aimed to obtain in vitro shoots by direct organogenesis from sorghum variety CIAP 2E-95, for the formation of calluses with embryogenic structures. Mature seeds were collected from plants grown under controlled conditions, which were disinfected and were placed in a germination medium with MS salts, myo-inositol 100 mg L^-1, 3% sucrose, pH 5.7, phytagel 2.5 g L^-1. Different concentrations of 6-benzylaminopurine (6-BAP) and kinetin were studied for shoot multiplication. Using 6 - BAP 0.22 mg L^-1 in the multiplication culture medium increased the number of shoots per explant, showing phenolic oxidation. Adding 50 mg L^-1 of ascorbic acid to the culture medium resisted phenolic oxidation and the number of sprouts (5.0), height and number of leaves increased significantly. Quality in vitro buds for callus formation were obtained stimulating the thickening by using MS salts, without growth regulators, ascorbic acid 50 mg L^-1, phytagel 2.5 g L^-1 and pH 5.7 and 40 g L^-1 of sucrose. The largest diameter of shoots, height, number of leaves and roots of in vitro plants, was achieved after 21 days of cultured in MS salts with 40 g L^-1 sucrose. Calluses formation with embryogenic structures from the central cylinder of curled leaves of the nearest segment to the base of thickened shoots was achieved after 45 days of culture. Segments from the central cylinder of the curled leaves are a safe source for the formation of embryogenic calli.

Keywords : 6-BAP; in vitro shoots; callus with embryogenic structures; kinetin.

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