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Revista Colombiana de Biotecnología

versão impressa ISSN 0123-3475

Resumo

PEREZ PEREZ, Jorge Liusvert et al. Influence of light type and intensity on induction and proliferation of somatic embryos in soybean. Rev. colomb. biotecnol [online]. 2012, vol.14, n.2, pp.139-146. ISSN 0123-3475.

The somatic embryogenesis represents an alternative for the regeneration of plants, in which influence diverse factors during the cultivation. Among these factors, the conditions of illumination during the induction of somatic embryos are determinant. However, works not exist that refer the effect of the sunlight on the formation of embryos in soybean. The goal of this work was to evaluate the effect of light type and intensity on the induction and proliferation of somatic embryos in soybean cultivar INCASoy-27. Immature cotyledons for 3,0-4,0 mm in length was placed on culture medium with 40 mg.l-1 2,4-diclorophenoxiacetic acid, sucrose 3%, and pH 7,0 under growth chamber with artificial light and range luminous intensity between 5-10 µmol.m-2.s-1 and 68-73 µmol.m-2.s-1 and growth chamber with sunlight from 50-65 µmol.m-2.s-1 were evaluated. The higher number of embryogenic cotyledons (95,31) was obtained into growth chamber with artificial light and intensity high. The results showed the major formation of embryos in artificial light (9,65) and sunlight (8,45) with luminous intensity high. They were not significant differences between two light types during somatic embryos multiplication. This is the first report on induction of somatic embryos in soybean with employment of sunlight as illumination source.

Palavras-chave : Tissue culture; somatic embryogenesis; photoperiod; Glycine max.

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