Abstract

LARA MANTILLA, Cecilia  and  ACOSTA PINEDA, Roberto Carlos. Isolated cellulolytic bacteria in the gut termites (Nasutitermes nigriceps) with probiotic characteristics and potential pasture degradation. Rev. colomb. biotecnol [online]. 2013, vol.15, n.1, pp.8-16. ISSN 0123-3475.

The objective of this research was to isolate cellulolytic bacteria in the gut of termites (Nasutitermes nigriceps) to determine their probiotic properties in vitro and its potential degradation of grass. Termites were treated with antibacterial detergent, separating and then macerating the intestine of the same in sterile peptone water. Dilutions of this mixture were inoculated in Petri dishes with Luria Bertani (LB) and carboxymethylcellulose (CMC) 2%, incubated at 37 ° C for 24 hours, and then revealed with 1% Congo Red. Bacteria that had higher degradation halos were subjected to tests and Gram stain probiotic temperature, pH, salinity and presence of bile salts, as well as antagonism tests and degradation of grass. The results revealed the presence of 9 bacilli cellulolytic Gram (-) of which the bacilli BTN7 and BTN8, showed the best degradation halos, 12 and 14 mm in diameter respectively, growing well in the different tests probiotic with densities between 10⁶ and 10⁸ CFU / ml, the degradation percentages of dry matter was 39.73% and 36.10% within 48 and 72 hours respectively. Biochemical tests revealed that API E (bioMérieux SA) bacilli BTN8 BTN7 and belong to the genus Enterobacter sp. The above results open the possibility of using these organisms as additives in feed for ruminants, in order to contribute to a better use of pasture, and other lignocellulosic plant substrates.

Keywords : Probiotics; Maralfalfa (Pennisetum sp.); ruminal microbes; lignocellulose; Enterobacter.

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