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Revista Colombiana de Biotecnología

Print version ISSN 0123-3475

Abstract

BASAIL PEREZ, Milagros et al. New alternative for micropropagation in temporary immersion system of plantain cultivar "INIVITPV-2011" (AAB). Rev. colomb. biotecnol [online]. 2013, vol.15, n.1, pp.98-107. ISSN 0123-3475.

This work was developed at the Plant Biotechnology Laboratory from the Research Institute of Tropical Root and Tuber Crops (INIVIT) in order to increase the multiplication coefficient in plantain cultivar "INIVITPV-2011" (AAB) in Temporary Immersion Systems (TIS). Different immersion times (10, 20 (control) and 30 minutes) and immersion frequencies (3, 6 (control) and 8 hours) in 10 L Nalgene flasks, and culture medium volume per explants (20, 40 (control) and 60 ml culture medium / explants) were studied, as well as, subculture time (15, 18, 21(control) and 25 days) and explants density per flask (20, 40 (control), 60 and 80 explants / culture flask). The multiplication culture medium MS supplemented with 2.0 mg.L-1 6-BAP, 3.5 mg.L-1 IAA, 30.0 gL-1 sucrose, 10.0 mg.L-1 ascorbic acid was used. Results obtained allowed to establish a methodology for micro-propagation of plantain cultivar "INIVITPV-2011" (AAB) in temporary immersion system, which consisted of using a 10 minute immersion time with 3 hour frequency (8 immersions per day). Besides, 60 explants were inoculated in each 10 L flask, and the renewal with 3600 ml culture medium and 18 day culture time allowed to obtain the highest productivity in the multiplication stage. These results, using the temporary immersion system allowed to establish a method for micro-propagation of plantain cultivar "INIVITPV-2011" (AAB).

Keywords : in vitro propagation; time; frequency; immersion; multiplication.

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