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Revista Colombiana de Biotecnología

Print version ISSN 0123-3475

Abstract

ESCUDERO AGUDELO, Janneth; DAZA MERCHAN, Zunny Tatiana; GIL ZAPATA, Nicolás Javier  and  MORA MUNOZ, Oscar Yesid. Evaluation of cellulolytic enzymes produced by native fungi through solid state fermentation (SSF) using sugarcane harvesting residues. Rev. colomb. biotecnol [online]. 2013, vol.15, n.1, pp.108-117. ISSN 0123-3475.

Sugarcane harvesting residues are considered as a raw material for fuel ethanol production due its high content of cellulose, around 40% DS. The use of cellulose depends of the application of physicochemical or biochemical treatments that allow the release of glucose and its subsequent uses in fermentation processes. The enzymatic hydrolysis of these residues requires a cellulolytic complex produced by microorganisms, including three enzymatic activities: Endoglucanases, β-Glucosidases and Exoglucanases. In the present study, cellulolytic enzymes produced by two native fungi Aspergillus spp., CH 2016 and CH 2001 was assessment, through of solid-state fermentation processes using as raw substrate RAC and pre-treated with organosolvents (delignified). Strain CH 2016 had the highest endoglucanase activity 11.0773 U/mL in the raw substrate on day seven of fermentation, the same strain, in the delignified substrate showed the highest activity exoglucanasa (0.042 U/mL ) and total cellulase (0.287 UPF/mL) on day five of fermentation. Strain CH 2001 got the highest β-glucosidase activity (0.1778 U/mL) in the substrate without pre-treatment on day 5 of fermentation. It was observed that the variables as substrate and fermentation time affected the expression of cellulolytic enzymes.

Keywords : solid State Fermentation SSF; Sugarcane harvest residues; Cellulose; Cellulolityc enzymes; Aspergillus spp.

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