Abstract

GUTIERREZ-IBANEZ, Ana Tarin et al. Detection of Ca Liberibacter solanacearum and phytoplasma in potato crop (Solanum tuberosum L.) in Toluca Valley. Rev. colomb. biotecnol [online]. 2013, vol.15, n.1, pp.145-149. ISSN 0123-3475.

In Mexico and Central America have been detected stained potato tubers with internal browning; recently in Texas, USA, this disease has been called "Zebra Chip" (ZC) or striped potato, foliar symptoms resemble the syndrome called "Potato Purple Top" (PPT) or "psyllid yellows" disease which is associated with the presence of "Candidatus Liberibacter solanacearum". The aim of the current work was to detect the presence of this bacterium and phytoplasma in potato plants with purple top symptoms. During 2011 and 2012 Spring - Summer cycle, a directed sampling was carried out in Tenango del Valle, Zinacantepec, Villa de Allende and San José del Rincón, State of México. The detection of both pathogens was performed by Polymerase Chain Reaction (PCR) with specific primers for phytoplasmas: P1/ P7, R16mF2/R16mR1 and for Ca Liberacter solanacearum: OA2/Oi2c, being 35,8 % from the positive plants for phytoplasmas and 11,6 % for this bacterium. These results indicated that in some areas these two PPT syndrome suspected causative agents, phytoplasmas and Ca. Liberibacter solanacearum, could be associated in the State of Mexico potato-producing region.

Keywords : Candidatus Liberibacter solanacearum; Phytoplasm; Purple Top.

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