Abstract

RAYAS, Aymé et al. Mannitol and silver nitrate effect of taro (Xanthosoma spp.) in vitro conservation. Rev. colomb. biotecnol [online]. 2013, vol.15, n.1, pp.167-171. ISSN 0123-3475.

Maintenance field genebanks are costly, in addition to the risks they face; to that effect on tissue culture is a solution to these problems. In vegetative propagated crops is desirable to use a combination of storage technology rather than relying on just one. in vitro culture provides an alternative for improving productivity and production of healthy planting material of taro (Xanthosoma spp.). This research was conducted in the Tissue Culture Laboratory of the Research Institute of Tropical Crops. Our objective was study the conditions for minimal growth conservation in vitro germplasm in this species. As plant material was used clone of Taro Xanthosoma 'INIVIT MX-2008'. The establishment of the plant material and its subsequent multiplication were carried out according to the methodology recommended by García et al. (1999). For the maintenance in culture of minimal growth basal medium MS was used and studied 15 treatments with combined concentrations of mannitol (osmotic regulator) (1.5, 3 and 4%) and silver nitrate (Ethylene inhibitor) (0, 2, 4, 8, 10 mg.L^-1). It concludes that it is possible to conserve taro Xanthosoma genetic resources in vitro, for over 10 months in a culture medium composed of MS salts and vitamins and supplemented with 4% mannitol and 4 mg.L^-1 of silver nitrate. Plants propagated from this culture medium were recovered successfully. The presence of higher concentrations of mannitol, may have influenced that increases survival of preserved material. O3

Keywords : minimal growth; genetic resources conservation.

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