Abstract

BAO FUNDORA, Lourdes et al. Somatic embryogenesis of Citrus macrophylla Wester using Pectimorf® and analogues of brassinosteroids. Rev. colomb. biotecnol [online]. 2013, vol.15, n.1, pp.189-194. ISSN 0123-3475.

Citrus fruits are widely used as rootstock. To increase the amount of these crops in plantations, in vitro propagation techniques such as somatic embryogenesis can be used, which requires artificial culture media and plant hormones. Due to the high cost of the plant hormone, a Cuban alternative is the use of cuban bioregulators growth as the analogs of brassinosteroids, 25(R) 2± , 3± , dihidroxi 5± espirostan- 6-ona (Biobras-6) y C: 25(R) 2± , 3± , 5± , trihidroxiespirostan-6-ona (MH-5) and oligogalacturonic mixed degree polimerization between 10-14 (Pectimorf ®). These bioregulators are effective in morphogenetic processes as a substitute or complement for auxins and cytokinins. Our work was aimed to determine the effect of Pectimorf ® and brassinosteroids (MH-5 and Biobras-6) in morphogenetic development and to obtain embryogenic cell line of Citrus macrophylla Wester. We used the medium of Murashige and Skoog (MS) (1962), supplemented with MH-5, Biobras-6 and Pectimorf ®. Embryos, roots and seedlings were obtained through somatic embryogenesis in all treatments. These products were effective in plant regeneration.

Keywords : in vitro culture; Citrus; growth bioregulators.

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