Abstract

PENARANDA, María Isabel; LIGARRETO, Gustavo Adolfo  and  NUNEZ, Víctor Manuel. Genetic transformation study in climbing pea Santa Isabel cultivar. Rev. colomb. biotecnol [online]. 2013, vol.15, n.2, pp.29-37. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v15n2.41264.

Different genetic transformation alternatives were tested in pea, "Santa Isabel" cultivar, with the purpose of studying the factors that affect the process. The methods of infiltration with vacuum, direct infection of the explants, pollen transformation and ovary microinjection were used. The hystochemical test of the gus expression was chosen as analysis method in the determination of positive transformants. With the used methodologies, blue spots in the plant tissue were detected, which indicates transient expression of the transgene in utilized explants. The obtained results suggest that the genetic transformation in pea genotypes planted in Colombia can be utilized for the introduction of genes of interest as support to genetic improvement.

Keywords : genotypes; gus expressions; plant breeding.

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