Resumo

GARCIA-GUTIERREZ, Cipriano; GONZALEZ-MALDONADO, María Berenice; MEDRANO-ROLDAN, Hiram  e  SOLIS-SOTO, Aquiles. Study of the mixing conditions in bioreactor for blastospores production of Beauveria bassiana. Rev. colomb. biotecnol [online]. 2013, vol.15, n.2, pp.47-54. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v15n2.35118.

In this work three fermenters were characterized: New Brunswick M-19 of 14 liters, Biocontroller Applikon 1035 of 7 liters and New Brunswick Bioflo III of 7 liters, determining the volumetric coefficient oxygen transfer (K_La), gas hold up (GH) and the mixing time (t_M). The fermenter New Brunswick Bioflo III had the best values with a ratio of diameter of the impeller/vessel (D_I/D_T) of 0.43, K_La = 9.5-208 h^-1 and t_M = 1.0-3.0 s, so it was selected for the production of blastospores of Beauveria bassiana, using molasses as carbon source. We studied the mixing conditions, using different combinations of impellers, type Rushton, Lightnin and Maxflo under a factorial experimental design 3². The propagation time was of 4 days, working volume 4l, 10% inoculum (1x10⁶ blastospores/ml), temperature 30°C, agitation of 400-500 rpm, aeration 0.5-1.0 vvm, and pH of 5.4. The fungus growth better using a combination of impellers Rushton-Maxflo at 400 rpm and 1.0 vvm (F = 10.324, p ≤ 0.0123) (LSD = 0.585) obtaining a concentration of 1.2x10⁹ blastospores/ml, 2.2 g/l biomass and 2.48 g/l of substrate consumption (Y x/s = 0.89). The mixing conditions and the parameters obtained can be applied to optimize the blastospore productions of B. bassiana to fermenter level in the experimental production of bioinsecticides.

Palavras-chave : liquid fermentation; aeration; agitation; entomopathogenic fungus.

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