Abstract

MURILLO GOMEZ, Paola A  and  ATEHORTUA, Lucía. Cell cultures of Choiba Dipteryx oleifera Benth. Rev. colomb. biotecnol [online]. 2013, vol.15, n.2, pp.124-131. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v15n2.36862.

Choibá (Dipteryx olifera) is a tree of the Fabaceae family, with a geographical distribution reported from Nicaragua to Colombia, nearly 1.000 msnm in a tropical rain forest. This species is a highly desired tree for its timber and fruits, the kernel store a lot of important oils for the food industry, resulting in a new possible food source, so we are making in vitro cultivation of vegetables with the purpose of producing compounds of interest and that mark a starting point on the reduction of land use and it achieves bioactive components under controlled conditions. In this work, as a first experimental step was evaluated the tissue cell growth in suspension, using fragments of cotyledon and testing 6 different treatments, the half of those with MS as basal medium and the other half with B5, each of the two groups with one control and hormone combination of 2.5 mg/L 2,4-D and 1 mg/L BAP or kinetin, supplemented with adenine, biotin, pantothenic acid and glutamine and 30 g l sucrose under complete darkness. It was found that two treatments with MS in combination with 2.5 mg/L 2,4-D and 1 mg/L kinetin or BAP were the best.

Keywords : biomass; cell cultures; choibá; Dipteryx oleifera.

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