Abstract

SUAREZ, Isidro E.  and  QUINTERO, Irma R.. Micropropagation of Stevia rebaudiana Bertoni, a natural sweetener, through pre existing meristem explants. Rev. colomb. biotecnol [online]. 2014, vol.16, n.1, pp.29-33. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v16n1.29420.

Stevia rebaudiana Bertoni leaves are source of stevioside and rebaudioside, non-caloric sweetener substances. Seed and cutting estevia propagation is difficult due to seed sterility and small size plant, respectively. To evaluate shoot proliferation, in vitro-established estevia shoots were cultured in ½ MS with five (0.0, 2.22, 4.44, 8.88 and 17.6 µM) BAP levels. Thereafter, proliferated shoots were cultured on ½ MS with five NAA levels (0.0, 2.69, 5.37, 10.74 and 21.48 µM) to evaluate shoot rooting. Finally, non-rooted shoots, in vitro-rooted shoots and non-rooted shoots treated with a 0.4% NAA powder were transferred to ex vitro conditions. All experiments were distributed using a complete randomized design. The data indicated that BAP treated shoots showed a higher rate of shoot proliferation. An 87% of rooting and higher number of roots per explant was achieved with 10.74 µM of NAA. Non-rooted shoots transferred directly from Stage II showed the best survival rate.

Keywords : Stevia; stevioside; micropropagation; plant growth regulator.

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