Resumen

ESQUIVIA MERCADO, Mabel Betariz et al. Ethanol production from cassava using very high gravity conditions (VHG). Rev. colomb. biotecnol [online]. 2014, vol.16, n.1, pp.163-170. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v16n1.44284.

This research exposes the fermentative medium and initial sugar concentration effect on ethanol production in batch systems, when used glucose syrups derivatives of cassava (Manihot esculenta), as a carbon source. The experimental design was factorial and analyzes the productivity of bioethanol in four different fermentation media, two of which were supplemented with cassava flour sugared. The initial sugar concentrations tested on these substrates were 250, 300 and 350 g / L. These fermentative treatments were inoculated with a commercial ethanolic variety of S. cerevisiae at a concentration of 0.05% (w / v). The process conditions included a final volume of 150 mL, incubation at 35 ° C for 48 hours , constant stirring at 150 rpm and pH between 4.0 and 4.5. The saccharified from this cassava variety showed be excellent substrate for the bioethanol production, achieving increases of 550% in terms of volumetric productivity over the control experiment, the above, allowed us to achieve final concentrations of ethanol of 14.7% v / v, yields product / substrate of 0.5 g / g, and productivities of 2.4 g / L / h. Most interesting is that these kinetic parameters were achieved with the simplest fermentation medium evaluated, the mean was compound of saccharified as a carbon source and as a sole source of nutrition, and its initial concentration of sugars was 250g / L; the above shows the ability of these substrates for ethanol production purposes.

Palabras clave : Bioethanol; cassava flour saccharified; very high-gravity fermentations.

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