Abstract

BARRIOS SAN MARTIN, Yaima; ACOSTA DIAS, Silvia; GONZALEZ HERNANDEZ, Francisca  and  HAYES GARCIA, Ranyer. Optimization of culture media QBP to the production of biomass of consortium BIOYAF. Rev. colomb. biotecnol [online]. 2014, vol.16, n.1, pp.171-176. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v16n1.37928.

The optimization of culture media with industrial purposes, in most cases, has been made through empirical trial and error procedures. Using different statistical methods original culture media can be optimized, in many cases, is possible to obtain a more productive media, at the same cost as the original one. The culture media QBP was optimized for the growth of the bacterial consortium BIOYAF, which can degrade petroleum hydrocarbons, using a Categorical Individual Factor Design to determine the working time, a Factorial Design (24) to determine working range of elements concentration and a Surface Response Design to optimize the concentrations. The response variables of the experiments used in this work were: wet mass, mass (UDO), viable's count, conductivity, pH and surface tension. The optimal time for growth of the consortium was of six hours. The optimized QBP culture media (ammonium phosphate: 3.19; magnesium sulphate: 0,04; yeast extract : 3,77; sucrose: 47,89) allowed that the biomass' production increases of 1,540 UDO to 3,082 UDO.

Keywords : Surface Response Design; bacterial consortium; optimum growth.

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