Resumen

BELTRAN PEDROZA, Diana Marcela  y  MESA LOPEZ, Neftalí. Dichloride of mercury as a disinfectant in the micropropagation of cumin (Aniba perutilis Hemsley). Rev. colomb. biotecnol [online]. 2014, vol.16, n.1, pp.203-209. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v16n1.44302.

Cumin, Aniba perutilis Hemsley, a species of economic importance for its strength, durability and excellent quality of its wood; from an ecological point of view it's important for the survival of species and animals such as macaws and guacharo. It is reported by Von Humboldt Institute as Critically Endangered (CR), mainly due to over-exploitation of forest redoubts where there are still some copies and limited knowledge of phenology and reproduction (Cardenas and Salinas, 2006). The use of micropropagation techniques is one of the methods of preservation, whereby it's necessary to overcome the induction phase where there is possibility of contamination of the explants. We developed a disinfection protocol which was achieved in vitro establishment of Aniba perutilis Hemsley. Initial disinfection was determined from the time of collection of the explants, which were placed in plastic boxes, previously disinfected with 96% alcohol, a solution of iodine + antifungal (Benomyl, 3grs/500 ml) called carrier solution. The best result in the contamination control was achieved using Tween 80 (1 drop / 100 ml) for 10 minutes and 20 minutes immersion in mercury dichloride (HgCl2) 0.2% with an 8% of contamination. Opposed to the treatment with sodium hypochlorite (NaClO), this showed an 80% contamination, for which one can attest that the mercury dichloride is an effective disinfectant for the control of fungal and bacterial contamination of the explants endogenous buds, both apical and lateral A. perutilis Hemsley.

Palabras clave : disinfection; in vitro culture; carrier solution; buds; contamination.

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