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Revista Colombiana de Biotecnología

Print version ISSN 0123-3475

Abstract

BARBOSA CEPEDA, Iván Darío  and  CHAPARRO-GIRALDO, Alejandro. Optimization of an in vitro regeneration system for colombian indica rice varieties. Rev. colomb. biotecnol [online]. 2014, vol.16, n.2, pp.19-29. ISSN 0123-3475.  http://dx.doi.org/10.15446/rev.colomb.biote.v16n2.38017.

In order to optimize an in vitro regeneration system for colombian rice varieties, the amount of plant regulators used for each step of the process was modified, together with other factors such as seed position and time of exposition to plant regulators. Modification of 2,4-D concentration during callus induction and coculture has a negative effect on regeneration potential. However, it is possible to increase the amount of produced calli by modifying the position in which the seed is placed on the culture medium. Moreover, a longer callus induction time decreased the regeneration percentage. During the regeneration step, for FEDEARROZ 2000 variety, a combination of 1,0 mg/l g/l mg/l of naftalenacetic acid + 4,0 mg/l g/l mg/l of kinetin increases the regeneration percentage, without increasing the amount of abnormal shoots and without decreasing their size. For FEDEARROZ 50 variety, the best combination of plant regulators is 0,5 mg/l g/l mg/l of naftalenacetic acid + 2,0 mg/l g/l mg/l of kinetin.

Keywords : Colombian rice; FEDEARROZ 2000 variety; FEDEARROZ 50 variety; plant regulators; somatic embryogenesis.

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