Abstract

CANALES, Pamela E.; CHAVEZ-HIDALGO, Elizabeth L.  and  ZAVALETA, Amparo I.. Characterization of halophilic bacteria producing amylase isolated from San Blas Salterns in Junin. Rev. colomb. biotecnol [online]. 2014, vol.16, n.2, pp.150-157. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v16n2.43763.

The aim of this study was to characterize halophilic amylolytic bacteria from San Blas Salterns-Junin, located in the Peruvian Andes at approximately 4 100 m of altitude. This study was conducted with 34 bacteria isolated from soil samples which were cultured in salt water medium (SW) 5 % containing 0,5 % yeast extract and 1 % starch. It was found that 41 % were starch-degrading bacteria, which were further characterized with conventional physiological and biochemical tests. Three bacteria were Gram-negative and eleven Gram-positive. Also, 21 % (3/14) was able to grow in a wide range of saltconcentration from 5 to 20 %. We reported that 14 % (2/14) of bacteria had all lipolytic, proteolytic and nucleolytic activity, and 29 % (4/14) had both proteolytic and nucleolytic activity. Bacteria were identified by restriction 16S ribosomal genes profiles, enzymes used were Hae III, BstU I, Hinf I and Cfo I. 16S ribosomal genes of seven isolated wich showed different DNA profiles were amplified, partial sequenced and analyzed using bioinformatic programs. By both phenotypic and molecular analysis of 14 amylolytic bacteria two groups were obtained, one belonged to the genus Halomonas (3) and the other, to the genus Bacillus (11). The characterized amylolytic bacteria could have a potential industrial use.

Keywords : San Blas Salterns; amylases; 16S ribosomal genes; ARDRA; Bacillus; Halomonas.

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