Abstract

FREIRE, Rodrigo; CARNEVALE, Nélida J.; ALZUGARAY, Claudia  and  BUENO, Mirian S.. In vitro culture of Schinus fasciculata (Griseb) J.M. Johnst var. fasciculata (molle). Rev. colomb. biotecnol [online]. 2014, vol.16, n.2, pp.169-173. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v16n2.35863.

Schinus fasciculata is a tree of Argentina Chaco eco-region. It is a low-growing species, used as an antiseptic, analgesic, anti-inflammatory and antifungal. Previous studies reported low germination and poor health. The object of this work was to evaluate the germination and in vitro response of seedlings in two culture media. Fruits were collected, disinfected for 20 min with HClNa and sowing in agar - water. The seedlings were seeded onto MS medium in two dilutions: fourth (¼ MS) and half (½ MS). Germination and vigor tests (mean time to maximum germination in days) were made. Contamination percentage was evaluated. At 20 days of transplanted were evaluated: % of explants with leaves, leaf number and height of seedlings. Parallel assay germination and vigor in sand was made. Contamination was 16%, germination was 84% and vigor 4.9 days. The 94 % and the 100% of the explants in MS ¼ and ½ MS showed leaves. The average leaf number was 7.5 and 7.4 and the average height was 3.35 cm and 2.98 cm in MS ¼ and MS ½, with a significant difference in height. Germination in sand was 46 % and vigor was of 4.6 days. The technique of in vitro culture in S. fasciculata facilitates the multiplication of this species of high pharmacological value, allowing to increase the percentage of germination and obtain pathogen-free all year seedlings.

Keywords : Schinus fasciculata; germination; vigor; in vitro culture.

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