Abstract

HINE GOMEZ, Ana; ROJAS VARGAS, Alejandra  and  DAQUINTA GRADAILLE, Marcos. In vitro establishment of two species native to Costa Rica: Terminalia amazonia (Amarillón) and Vochysia allenii (Botarrama Blanco). Rev. colomb. biotecnol [online]. 2014, vol.16, n.2, pp.180-186. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v16n2.47240.

The objective of this research was to achieve the in vitro establishment of the species Terminalia amazonia and Vochysia allenii due to the difficulty propagate sexually and asexually with conventional techniques. It was possible to establish the nodal segments of both species in in vitro conditions using 0.1 % HgCl2 with an exposure time of 5 minutes. The best nodal culture medium was 100 % WPM salts in T. amazonia and V. allenii was WPM 50% salts. After 28 days of culture 42 % of nodal segments to T. amazonia and V. allenii 10% was obtained. In both species, the effect on sprouting of five concentrations of 6-benzylaminopurine (6-BAP) (0.0, 2.22, 4.44, 6.66, 8.88 μM L-1) and five thidiazuron (TDZ) (0.0, 0.22, 0.45, 0.68, 0.90 μM L-1) was evaluated. Outbreak was scored on average per explant in the five treatments of BAP and TDZ used.

Keywords : in vitro culture; nodal segments; 6-benzylaminopurine; thidiazuron.

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