Abstract

PRIETO-CORREAL, Gloria Carolina; PRADA-SALCEDO, Luis Daniel; CUERVO PATINO, Claudia Liliana  and  FRANCO-CORREA, Marcela. Evaluation of organic acid production by Streptomyces spp. and solubilization of three phosphorus sources by strain T3A. Rev. colomb. biotecnol [online]. 2015, vol.17, n.1, pp.110-121.  Epub July 13, 2021. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v17n1.41667.

Fifteen isolates of Eastern Cordillera of the Colombian Andes were identified by morphological characteristics and 16S rDNA gene sequence. The BLAST analysis of 15 sequences shows that isolates belong to Streptomyes. Also we detected the organic acids in the solubilization process mainly oxalic acid, citric acid and gluconic acid. Streptomyces sp. (T3A) was selected in preliminary qualitative and quantitative assays by the high phosphorus solubilizing activity; in this work we evaluate this strain with different forms of inorganic phosphate. The results evidenced the capacity of this actinobacteria to solubilize phosphorous showed 122 mgP •L-1 Ca3(PO4)2, 14 mgP •L-1 AlPO4 and 19,6 mgP-L-1 for rock phosphate. Also the assays revealed that the activity was maintained between a pH range of 5 to 8 with the same sources of insoluble phosphates evaluated. These results contribute to characterize these strains as plant growth promotion bacteria and as key source in agricultural biotech.

Keywords : Streptomyces; phosphate solubilization; organic acids; actinobacteria; HPLC.

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