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Revista Colombiana de Biotecnología

Print version ISSN 0123-3475

Abstract

CANCINO-ESCALANTE, Giovanni Orlando; QUEVEDO GARCIA, Enrique; VILLAMIZAR, Carmen Edilia  and  DIAZ CARVAJAL, Claudia. Propagation in vitro of selected materials of Rubus glaucus Benth (mora de Castilla) in the province of Pamplona, northeastern Colombia. Rev. colomb. biotecnol [online]. 2015, vol.17, n.2, pp.7-15. ISSN 0123-3475.  http://dx.doi.org/10.15446/rev.colomb.biote.v17n2.54262.

In this study plant materials were selected in 53 farms belonging to four growers associations of blackberry in the municipalities of Pamplona and Chitagá (North of de Santander, Colombia). Nodal segments were used as initial explants of R. glaucus. For the establishment stage Murashige and Skoog, 1962 (MS) media was used and, supplemented with of gibberellic acid (GA3) (0.0 -0.1 mg/L) and 6-aminopurine (BAP) (0.0 -2.0 mg/L); for the multiplication stage MS was supplemented with GA3 (0.0 -0.03 mg/L) and BAP, (0.0 -2.5 mg/L) and for the rooting stage MS was supplemented with acid indolbutirico (0.0 -1.0 mg/L). From the data generated during the three stages, an experimental design of incomplete blocks was randomly applied and the treatments averages were statistically analyzed using the Tukey Test. The results indicated average rates of contamination (16.5-49.7 %), multiplication (3.8-4.3 shoots/explant) and in vitro rooting (3.3-4.3 roots/plant) for the different evaluated materials. These results, achieved first in the Northeastern region of Colombia, are important in that they will feature selected materials available for blackberry growers in the region.

Keywords : Andean blackberry; micropropagation; growers associations; 6-aminopurine.

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