Abstract

RUIZ, Carolina; GOMEZ, Martha  and  VILLAMIZAR, Laura. Formulation prototype and atmosphere packaging for the antagonistic strain Pseudomonas fluorescens PS006. Rev. colomb. biotecnol [online]. 2015, vol.17, n.2, pp.93-100.  Epub Aug 09, 2021. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v17n2.54282.

The isolation Pseudomonas fluorescens PS006 demonstrated high potential to be used as an active ingredient of a bio-produt, because its capacity to produce biosurfactants, its phosphorus solubilizing activity and its antagonistic activity over different phytopathogens. For this reason, the present work had as objectives to develop and characterize a formulation prototype based on P. fluorescens PS006, stable under storage conditions. Initially the active ingredient was characterize and compatible formulation auxiliaries, were selected evaluating the stability of its mixture with three solid diluents at two different moistures (10% and 20 %) during three months of storage at temperatures of 8, 18 and 28 ± 2 °C. The active ingredient showed in vitro biocontrol activity over four phytopathogens and temperature and humidity affected its stability during storage. Stability of the most stable formulation prototypes in terms of viability and biocontrol activity was evaluated in presence and absence of oxygen and membrane protectors. Support S1 with 20% of moisture mixed with the active ingredient without addition of membrane protectors and stored in presence of oxygen, was selected as the most stable treatment during six months of storage at three temperatures, with viability losses lower than 5%.

Keywords : biological control agent; biocontrol; formulation; Pseudomonas spp; shelf-life.

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