Abstract

PEREZ-MARTINEZ, Belkys Adriana. Establishing in vitro Hypericum goyanesii Cuatrec. E Hypericum juniperinum Kunth, from seeds culture. Rev. colomb. biotecnol [online]. 2015, vol.17, n.2, pp.156-160.  Epub Aug 11, 2021. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v17n2.54294.

The purpose of this study was to evaluate germination culture media for the in vitro establishment Hyperi-cum goyanesii e Hypericum juniperinum as ex situ conservation strategy for biotechnology of plant species belonging to the high-Andean ecosystem. The seeds were disinfected with sodium hypochlorite and seeded into six treatments MS and MS with 50% reduction of its macro and microsales and vitaminas, with and without the addition of activated charcoal and pulp banana. Germination is favored by the use of banana pulp in MS medium with reducing salts and supplemented with activated charcoal. It was possible to show that sexual explants and H. goyanesii e H. goyanesii juniperinum were influenced positively variable germination percentage, by the use of organic substances to replace growth regulators.

Keywords : Hypericaceae; in vitro culture; germination; disinfection; culture medio.

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