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Revista Colombiana de Biotecnología

Print version ISSN 0123-3475


SANCHEZ LOPEZ, Diana Beatriz; PEREZ PAZOS, Jazmín Vanessa  and  DAVID HINESTROZA, Hilda Adriana. PGPB effect on the growth Pennisetum clandestinum under salt stress. Rev. colomb. biotecnol [online]. 2016, vol.18, n.1, pp.65-72. ISSN 0123-3475.

This research aims the isolation and characterization of bacteria with potential to promote the growth of grass Pennisetum clandestinum in simulated saline soils. 92 Gram positive bacterias were isolated from Rhizosphere soil samples of P. clandestinum. Ten isolated bacteria grew on nutrient agar supplemented with NaCl (2.578 M). Isolates were evaluated under greenhouse conditions; the strains identified as KISA 34 and KISA 71 were selected as the best based on the statistical test of Dunnet (p ≤ 0.05) and were identified Bacillus amyloliquefaciens KISA 34 and Bacillus subtilis KISA 71. These strains have the ability to produce ammonium, exopolysaccharides and cellulose, both in absence and presence of NaCl. The strains produced indoles and phosphorus solubilization. The evaluation of strains in greenhouse showed that the T6 - KISA 34 + KISA 71+ 25 % (phosphate rock RF) significantly increased biomass and plant development (p≤ 0.05) compared with T2 - full fertilization . The results of this research showed that isolates have the ability to grow in saline soils retaining its characteristics as promoting plant growth with positive effects on P. clandestinum.

Keywords : Bacillus subtilis; Bacillus amyloliquefaciens; biofertilizer; salinity; phosphorus solubilization.

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