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Revista Colombiana de Biotecnología

Print version ISSN 0123-3475


GIRALDO MARROQUIN, Natalia; BUSTAMANTE RODRIGUEZ, Silvia Lizette; PINZON GUTIERREZ, Yeimy Alexandra  and  BUITRAGO HURTADO, Gustavo. Molecular characterization of Colletotrichum gloeosporioides isolated from yam plants of Colombia Atlantic Coast using DNA Amplification Fingerprinting technique (DAF). Rev. colomb. biotecnol [online]. 2016, vol.18, n.1, pp.95-103. ISSN 0123-3475.

Yam anthracnose, caused by the fungus Colletotrichum gloeosporioides, is a significant disease that has the potential to destroy harvests in 100%, causing the decrease in crop yields nationally. Consequently, its situation affects approximately 35.000 families of small farmers the Atlantic Coast for whom it is the livelihoods. Due to above, the objective of this work was to perform the molecular characterize of 42 C. gloeosporioides isolates from yam plants with symptoms of the disease, using the molecular technique "DNA Amplification Fingerprinting (DAF)" useful for its resolution in determining the genetic variability of different organisms. For polymorphisms determination, were amplified 16 DAF markers by using primers decamers type, the banding patron was visualized by electrophoresis in microchip 202 MCE-MultiNA equipment also, we assessed of DAF technique reproducibility. The amplification process yielded 391 unequivocally polymorphic bands in all samples, Through Dice coefficient we identified five groups with 0.30% of similarity and a genetic diversity index of 0.28. Our data show a high degree of variability in the collection of C. gloeosporioides studied. The high variability could be due to several reasons like germplasm exchanges, the heterothallic condition of fungal isolates, a presence of mutations and the high potential for conidia dispersal that allow it to maintain its viability under adverse conditions. Finally, we found that DAF technique is reproducible, confirming that it is a reliable methodology for the fungi molecular characterization.

Keywords : anthracnose; necrosis; genetic diversity; DNA; fungi.

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