Abstract

BAUTISTA, Jessica P; BARBOSA, Helber  and  URIBE-VELEZ, Daniel. Formulation prototype based on Rhodotorula mucilaginosa for the control of Botrytis cinerea in Roses. Rev. colomb. biotecnol [online]. 2016, vol.18, n.2, pp.13-23. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v18n2.55826.

Productive systems of cut roses for exportation, have important challenges due to the presence of various plant pathogens in such crops. Botrytis cinerea is one of the most important pathogen microorganism because of its persistence and number of alternative hosts plants. International markets are very demanding in terms of environmentally sustainable crop management, which has forced for the implementation of strategies for biological control of diseases. The phyllosphere yeast Rhodotorula mucilaginous (Lv20) with biocontrol potential against B. cinerea, was employed in this study in order to generate a solid prototype formulation to achieve both, the stability of its activity and cell viability over time. The use of the mix of synthetic and naturally occurring polymers allowed to maintain the viability of this strain for 90 days at 1,90x10⁹ células.mL^-1 at a 25 ° C in a liquid formulation. Likewise, the prototype formulation using mannitol as a nucleating agent in a granular solid formulation, allowed obtaining a cell viability of 1.2x10⁸ células.gr^-1 after 90 days of storage at 4 °C, maintaining a biocontrol activity equal to the fresh strain with or without formulation. These results allow us to suggest that the formulation prototypes using R. mucilaginous yeast, is a promising alternative for the control of B. cinerea in post-harvest management of roses.

Keywords : Yeast; grey mold; solid formulation; biopolymers; biological control; postharvest.

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