Resumo

VACA-VACA, Juan Carlos et al. First report of a begomovirus presents in yellow passionfruit [Passiflora edulis f. flavicarpa (Degene)] in Valle del Cauca, Colombia. Rev. colomb. biotecnol [online]. 2016, vol.18, n.2, pp.56-65. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v18n2.52904.

The yellow passion fruit is a crop of economic importance to the Valle del Cauca, but in recent years has been a reduction of up to 80% in production due to viral problems. The aim of this study was to detect and characterize partially begomovirus that could be affecting crops of yellow passionfruit located in two growing areas of the Valle del Cauca (La Union and Palmira). Passionfruit leaves with typical symptoms of viral disease were collected, purified genomic DNA and its bipartite begomovirus were detected by PCR using primers specific for component A and B, respectively. Five DNA fragments corresponding to geminiviral A and B genome were cloned, sequenced and analyzed with bioinformatics tools. The results show for the first time in Colombia the presence of a begomovirus in yellow passionfruit. The analysis of the nucleotide sequences indicates that this begomovirus is more related to bean BDMV, a geminivirus affecting beans in Colombia, not PSLDV, which was reported affecting yellow passionfruit in Brazil. To date, this result would be the first report of a begomovirus (belonging to the family Geminiviridae) affecting yellow passion fruit in Colombia, and that might be different from other begomovirus previously reported worldwide affecting this crop.

Palavras-chave : Geminivirus; Passiflora edulis f. flavicarpa; PSLDV.

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