Abstract

CISNEROS R, Carlos A; SANCHEZ DE P, Marina  and  MENJIVAR F, Juan Carlos. Identification of phosphate solubilizing bacteria in a Andisol of Colombian coffee region. Rev. colomb. biotecnol [online]. 2017, vol.19, n.1, pp.21-28. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v19n1.65966.

Phosphorus is an essential nutrient for coffee growing, however, in Colombia most of the soils have low concentrations of this element. A strategy to supply the demand is the use of phosphate solubilizing microorganisms (PSM), in that order, 26 rhizospheric bacteria of Typic melanudans soil of Cajibío (Cauca, Colombia) were isolated, in three agroecosystems: coffee at full sun exposure, coffee with shade and relic secondary forest, evaluating the phosphate solubilizing efficiency (PSE) for Ca-P, Al-P, Fe-P in the solid and liquid Pikovskaya media. It showed the following solubilization sequence: Ca-P> Al-P> Fe-P. Two isolated bacteria were identified by DNA extraction and analysis of the 16S rRNA gene as Kocuria sp, and Bacillus subtilis. Later, using HPLC were identified organic acids present in the three phosphorus sources as: citric, gluconic, D- and L-malic, D- and L-lactic acid with higher presence in Ca-P-Kocuria sp. Strong acidification was observed for Fe-P and Al-P in all conditions.

Keywords : Bacillus subtilis; Kocuria sp; biofertilizers; phosphate solubilizing efficiency; organic acids.

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