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Revista Colombiana de Biotecnología

versão impressa ISSN 0123-3475


ARISTIZABAL-VASQUEZ, Darly Yuliana et al. Effect of Tween ® 80 on calcium carbonate bioprecipitation mediated by Bacillus cereus. Rev. colomb. biotecnol [online]. 2017, vol.19, n.1, pp.116-123. ISSN 0123-3475.

This research presents the sprinkling effect of Tween® 80 for three concentrations (0.00% w, 0.25% w y 0.50% w) in a processes of calcium carbonate biosynthesis. The assays used a culture of Bacillus cereus, isolated from the gardens of the Universidad Pontificia Bolivariana (Medellin, Colombia). The experiments were monitored for 6 days measuring pH as well as through mineralogical analyses for precipitates using scanning electron microscopy, X-ray diffraction and Fourier transform infrared spectroscopy. Tween® 80 had an important role as deagglomerate, decreasing the precipitation of biomineralized-carbonate structures (generated by bacteria). It was also found that the evaluated concentrations did not inhibit the bacterial growth. Additionally, Tween® 80 favored the production of vaterite, increasing its ratio in comparison to calcite (around 98% by using 0.50% w of Tween® 80).

Palavras-chave : Tween® 80; biomineralization; Bacillus cereus; calcite; vaterite.

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