Resumo

PANTOJA GUERRA, Manuel; MENDOZA REYES, Sandra  e  VALERO VALERO, Nelson. Design of culture medium for biomass production of Microbacterium sp. (BSC3) to the humified organic matter generation from lignite. Rev. colomb. biotecnol [online]. 2018, vol.20, n.1, pp.31-41. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v20n1.62764.

A culture medium for the multiplication of a coal solubilizing bacterial strain was designed (BSC3); were used three agroindustrial wastes: cane molasses, whey and crushed rice. Through Box-Behnken surfaces responses designs, two concentration ranks (2-10% y 0-6%) were evaluated, so the adequate combination to BSC3 biomass production was found. Bacterial growth curves were constructed to determine some kinetics parameters (growth specific rate [u], duplication time [Dt] and biomass final production), that were compared with the strain growth in a control culture medium; the optimized culture medium was also elementally caracterizated. The optimal concentrations to BSC3 biomass obtaining were: 6% molasses cane, 2,5% whey plus a salts minimum medium, with pH of 6,5. The kinetics parameters in this medium were: final biomass = 3,2 g.L-1, u=0,0206 h-1, Dt=33,64 h, and in the control medium: final biomass = 3,4 g.L-1, u=0,0139 h-1, Dt=49,85 h, this shows that the optimized medium allows the increase of the specific growth rate and a less duplication time of BSC3, so the optimized medium allowed to conserve its coal solubilizing activity.

Palavras-chave : Whey; cane molasses; Microbacterium sp.; coal solubilizing.

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