Abstract

HERNANDEZ-FERNANDEZ, Javier; PEREZ, Elia Elizabeth  and  PINEROS-CASTRO, Yineth. Identification and screening of lignocellulosic activity in native isolates of Trichoderma spp., obtained from palm oil biomass. Rev. colomb. biotecnol [online]. 2018, vol.20, n.1, pp.59-67. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.b¡ote.v20n1.73693.

Previously, there were isolated eight native strains of Trichoderma sp. from a cluster of decomposing oil palm from Cumaral, Meta, Colombia. The ITS1-ITS4"s region was used for the molecular identification and the cellulase activity (filter paper activity) of the complex produced by strains was determined using palm waste as substrate. Seven native isolations showed between 97-100% similarity with fungi of the genus Trichoderma. It was observed for seven of the eight strains the presence of the five anchors which identify fungi of the genus Trichoderma, finding five of the native fungi such as Trichoderma koningiopsis (HR-04-89; HR-11-89; HR-19-89; y HR-06-89) four as Trichoderma asperellum (HR-01-89; HR-03-89; HR-16-89; HR-18-89). The bioassay showed that Trichoderma strains tested are statistically significant on the enzymatic activity of cellulases on filter paper (p <0.05). In addition, strains HR-01-89, HR-03-89, HR-11-89, HR-04-89 and HR-18-89 showed no differences in enzymatic activity. The reference strain used, Trichoderma reesei produce a superior and different behavior compared with the native strains. The native strain HR-18-89 (Trichoderma asperellum) had higher levels of enzyme activity, 78% of the value of the reference strain. It is important to identify and evaluate native strains of Trichoderma sp. with innovative biological activities that allow to degrade the recalcitrant cellulose of the African palm clusters.

Keywords : ITS1-ITS4; monospore crops; PCR; cellulases; Trichoderma.

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