Abstract

RAMIREZ-BABATIVA, Daniel Ferley  and  ESPINOSA RAMIREZ, Adriana Janneth. Comparison of the populational characteristics of Lemna minuta (ARACEAE: LEMNOIDEAE) in three culture media. Rev. colomb. biotecnol [online]. 2018, vol.20, n.1, pp.84-96. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v19n2.50511.

Although neotropical macrophytes are considered appropriate for diverse applications due to their great reproductive capacity and high sensitivity to changing environmental conditions, research on these plants is currently scarce, especially in Colombia when compared to countries such as Brazil. The current research work intended to acclimatize and cultivate a clone of the duckweed Lemna minuta, which is widely distributed in Colombia and America. After keeping daughter fronds of this species for two months in APHA culture medium, their propagation was compared in three culture media: Hoagland's E+, APHA and AAP20x. Population growth variables such as growth rate, mortality, doubling time and life span. Additionally, the efficiency of the frond cleaning method proposed by Acreman to obtain axenic cultures was evaluated. The results indicated that Hoagland's E+ medium (without organic compounds) is the most suitable one when it comes to frond growing under laboratory conditions, due to its associated higher frond production rate (0.16 fronds-d-1) and life span (13.8 d), as well as lower mortality (0.11 fronds-d-1) and doubling time (4.61 d). Knowing the population growth and cultivation conditions of L. minuta allows proposing it as a relevant macrophyte and candidate for various water quality bioassays.

Keywords : culture media; growth rate; propagation; duckweed; Lemna minuta.

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