Resumo

ARIAS, Duverney Gavina; AGUDELO, Alexander Guevara  e  LOPEZ, Emmanuel Cano. Evaluation of the growth of human fibroblasts on fibroin scaffolds from Bombyx mori L. Rev. colomb. biotecnol [online]. 2018, vol.20, n.2, pp.47-56. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v20n2.77062.

The fibroin of Bombyx mori L, has been used for its physical / chemical characteristics as a biomaterial with applications in the healing of multiple tissues. In the context of regenerative medicine, characterizing at a physical and biological level new supports prepared from silk fibroin and evaluating their capacity for the proliferation of human fibroblasts, offers a very attractive opportunity to find new biomaterials with favorable applications in the healing of wounds. 17% regenerated fibroin was used for the manufacture of matrices. The matrices constructed were characterized in aspects such as: stability under growing conditions, ultra-structure, porosity, contact angle and mechanical properties. Finally, the ability of the scaffold to support the growth of human fibroblasts was evaluated. The average thickness of the fibroin matrices was 30.1 µm, with a stability greater than 4 weeks under culture conditions, porosity of 51% and a liquid retention capacity of 95%, a contact angle of 44.5 ° and a modulus of elasticity of ± 200 MPa. It was identified that the scaffolds allow cell multiplication, showing low cytotoxicity indexes (<5%); the cells established strong interactions with the scaffold, through the production of filopodia and the production of their own extracellular matrix. Constructed scaffolds allow the multiplication of human cells with low cytotoxicity and these establish a close relationship at the cellular level with the biomaterial. This can be used as a compatible scaffold for the growth and multiplication of human cells in a regenerative medicine process of epithelial tissue.

Palavras-chave : biotechnology; biomaterial; tissue engineering; regenerative medicine.

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