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Revista Colombiana de Biotecnología

Print version ISSN 0123-3475

Abstract

RAMIREZ, Edilene; SZUREK, Boris  and  LOPEZ CARRASCAL, Camilo Ernesto. Factors affecting the transient expression of gene GUS in cassava (Manihot esculenta Crantz). Rev. colomb. biotecnol [online]. 2018, vol.20, n.2, pp.57-67. ISSN 0123-3475.  http://dx.doi.org/10.15446/rev.colomb.biote.v20n2.77063.

Transient expression is a common technique used for the co-expression of proteins for a wide range of experiments in molecular biology. This technique has been frequently used for gene study in plant pathogen interaction and has proved to be versatile and effective. However, there are still few reports and sparse data for transient expression in cassava leaves from adult/mature plants. For this reason, in this study we evaluated some regards of transient expression for the gene GUS in cassava: bacteria introduction methods, type of Agrobacterium strains, time post-inoculation, introduction of gene VirG and GUS and their expression into different cassava varieties. The results show that GUS expression is more efficient between 5-7 days post-inoculation with the GV3101 strain, moreover the virulence of this strain increases with the VirG gene. Finally, we observed a range of GUS expression pattern between different cassava varieties, altogether showing that the genetic factor is important for an accurate and effective agroinfiltration in cassava.

Keywords : Agrobacterium tumefaciens; beta-glucuronidase; VirG; agroinfiltration; cassava.

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